How do you make a 5% cell suspension

2 Add 2 drops of whole blood or 1 drop of packed cells into the appropriate labelled tube. 7.1. 3 Add 0.5 to 1.0 ml of saline to the labelled tube to produce a 3-5% red cell suspension.

How do you make a 5% cell suspension? - Google Search

Isotonic saline can be used as a diluent or PBS pH 7.0. 1 drop of whole blood in 49 of diluent, or 1 drop of packed red cells in 24 of diluent will give a 2% suspension.

How do you prepare pooled cells?

Preparation of Pooled Cell Suspension: Place 1 drop of red cells each from 3 of O group sample tubes or segment into the O labelled tube. Fill the tube ¾ full with 0.9% saline to resuspend the cells. Centrifuge the tubes for at least 2 to 3 minutes on high speed. Decant the supernatant fluid.

How do you prepare a 3% RBC suspension?

Dispense 2 drops of whole blood (or equivalent: 1 drop of packed cells) in the labelled tube. Add 0.5 to 1.0 mL of normal saline and mix to resuspend to 3%. Compare the colour visually with a 3% commercial red cell suspension and adjust the suspension strength if necessary.